![]() Drain isobutanol and wash the surface with distill water. Allow gel to polymerize within 10-30 minutes.Ī) When the gel has polymerized a distinct interface will appear between the separting gel and the isobutanol. Stop the pouring when gel solution reach to about 6 cm height and layer about 0.5 ml isobutanol on top of the separating gel solution to keep gel surface flat. Use 10-20% gel for low molecular weight (MW100kD) protein identification.Ī) After adding ammonium persulfate and TEMED immediately mix the gel solution gently and carefully introduce solution into gel casting chamber. Use gradient gel former to make gradient gel. Ammonium persulfate and TEMED should be added before pouring gel.Ī) For making 12 linear slab gels (ml) Separating concentrationī) For making 12 gradient gels (ml): Separating concentration
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